lowered DNA-binding affinity. The binding of GATA2 mutants for the PROX1 1 kb GATA web page was next investigated working with EMSA and recombinant purified C-terminal zinc finger constructs (Figure 2A). EMSAs demonstrated that the hematological GATA2 mutant R362Q will not significantly influence binding to DNA, whereas the hematological GATA2 mutants T354M and R398W bound with moderately lowered