lowered DNA-binding affinity. The binding of GATA2 mutants towards the PROX1 1 kb GATA site was subsequent investigated employing EMSA and recombinant purified C-terminal zinc finger constructs (Figure 2A). EMSAs demonstrated that the hematological GATA2 mutant R362Q will not significantly influence binding to DNA, whereas the hematological GATA2 mutants T354M and R398W bound with moderately lower